Open Conference Systems, INTERNATIONAL MULTIDISCIPLINARY CONFERENCE (IMC 2017) PROGRAM

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THE APPLICATION OF ANTIBODY COATED PAPER STRIPS IN BLOOD TYPING
Mohd Jaamia Qaadir, Darishini Sugumar, Mohd Sulaiman Nordin

Last modified: 2017-01-05

Abstract


This project was designed to develop a paper based rapid ABO blood typing method. The paper was soaked in antisera A and antisera B to trigger agglutination when blood droplets were placed on the treated paper. Hence, three objectives were analysed in this experiment. We first targeting to coat the antibodies onto the paper strips. All the paper strips were soaked into antibody solutions of different concentrations. Secondly, the demonstration of typing ABO blood groups were done by using the antibody coated paper strips. Thirdly, the determination of the strength of blood agglutination with the antibody coated paper strips. The data was analysed as descriptive data. A wicking distance determines the strength of agglutination. Each box in the strip represented strength of reaction with four (+), three (+), two (+) and one (+) from centre to both direction. A positive result demonstrated the strongest agglutination that graded four (+) while a negative result demonstrated with blood covering towards the end of the strip. The occurrence of blood chromatographic separation is based on the specific antibody- antigen interactions. Blood agglutination and wicking action on the paper strips followed the concepts of colloids chemistry. The immunoglobulin M antibodies agglutinated the red blood cells by polymer bridging, upon selective adsorption on the specific antigen at their surface.



Keywords


ABO blood typing, antibody, antigen

References


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